ABSTRACT
<p><b>OBJECTIVE</b>To obtain the optimal conditions for separating the alkaloids from the extract of Radix of Zanthoxylum nitidum by selecting appropriate macroporous adsorption resins.</p><p><b>METHOD</b>Eight types of macroporous adsorption were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of Alkaloids as indexes.</p><p><b>RESULT</b>The XDA-5 macroporous adsorption resin had the best separating efficiency. After enrichment and purification with it, the product purity and yield of alkaloids were up to 33.25% and 90.15%, respectively.</p><p><b>CONCLUSION</b>This method is simple, feasible and fit for industry production.</p>
Subject(s)
Adsorption , Alkaloids , Chemistry , Porosity , Resins, Plant , Chemistry , Zanthoxylum , ChemistryABSTRACT
<p><b>OBJECTIVE</b>Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease. It is characterized by selective loss of spinal cord motor neurons leading to muscle atrophy and is the result of mutation or deletion of the survival motor neuron (SMN) gene. Currently, there are no effective therapies for this disease. Stem cell therapy is a new prospect for SMA patients. This study aimed to investigate whether mesenchymal stem cells (MSCs) can be differentiated into neuron-like cells (NLCs) in SMA patients in order to provide a basis for stem cell therapy for SMA.</p><p><b>METHODS</b>SMA was definitively diagnosed using polymerase chain reaction-restriction fragment length polymerphhism (PCR-RFLP). Two children without SMN1 gene deletion were used as controls. MSCs were isolated and purified from SMA patients and controls, and induced into NLCs by bFGF and baicalin. The NLCs were identified by immunofluourescence staining with NSE and NF monoclonal antibodies.</p><p><b>RESULTS</b>SMA patients showed the deletion of SMN1 exon 7. The morphous and proliferative speed of MSCs between SMA patients and controls were similar. After 6-day induction, MSCs of the two groups displayed similar morphology to that of neurons, with long processes forming extensive networks. NSE and NF, the neuronal markers, were detected in the differentiated NLCs of the two groups.</p><p><b>CONCLUSIONS</b>SMN1 deletion appears not to affect the proliferation and differentiation of MSCs. MSCs of SMA patients can be differentiated into NLCs.</p>
Subject(s)
Adolescent , Child , Female , Humans , Male , Cell Differentiation , Cell Proliferation , Mesenchymal Stem Cells , Cell Biology , Muscular Atrophy, Spinal , Pathology , Neurons , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To explore the optimal separation of the alkaloids from Lotus plumule by selecting appropriate macroporous adsorption resins.</p><p><b>METHOD</b>To evaluated the separating efficiency by measuring the adsorption ratio, eluting ratio and the concentration of neferine.</p><p><b>RESULT</b>The LSA -5B macroporous adsorption resin prolided the optimal separating efficiency. The best adsorption capacity was achieved with the following conditions: the concentration of extact liquid was 0. 125 g x mL(-1) (equivalence to raw material), then the resin was washed by water to remove impurity and alkaloids were desorbed by 50% ethanol.</p><p><b>CONCLUSION</b>This method is simple, feasible and suitable for industry production.</p>
Subject(s)
Adsorption , Benzylisoquinolines , Drugs, Chinese Herbal , Nelumbo , Chemistry , Plants, Medicinal , Chemistry , Resins, Synthetic , Chemistry , Seeds , Chemistry , Technology, Pharmaceutical , MethodsABSTRACT
OBJECTIVE@#To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.@*METHODS@#The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.@*RESULTS@#Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).@*CONCLUSION@#The method may be used for the determination of diltiazem hydrochloride injection.
Subject(s)
Calcium Channel Blockers , Chromatography, High Pressure Liquid , DiltiazemABSTRACT
<p><b>OBJECTIVE</b>To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.</p><p><b>METHOD</b>A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).</p><p><b>RESULT</b>This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.</p><p><b>CONCLUSION</b>The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.</p>